Light Scattering applications

electrophoretic light scattering imageThe variety of dissolved or suspended particles that can be characterised by photon correlation spectroscopy and electrophoretic light scattering is enormous. They include nanoparticles, polymers, carbohydrates, proteins and micelles, to name but a few.

The value of a method which provides quick and accurate information on particle size and concentration need hardly explained. Zeta potential is a much more complex parameter.

It has a direct relation to solution stability, chemical modification and even biocompatibility, and is therefore an important factor in many nanoparticle synthesis and application studies.

As an example, if both the size and the zeta potential of a micelle are known, predictions can be made on how it might perform when applied to its intended end use.

Thinking of zeta potential as effective charge in solution, it is clear that the type of solvent used will directly affect the zeta potential produced. It is therefore essential that any instrument used should have the flexibility to accommodate a wide variety of both particles and solvent conditions.

By combining measurement of size and zeta potential in one instrument, Beckman Coulter has done much to reduce costs and increase efficiency in applications which require both. This is particularly useful in the study of micelles and liposomes, for instance.

Customised solutions are available for measurement of zeta potential in a wide variety of specialised applications. They include instruments with cells specially designed for solvents or for flat surfaces or films.

One popular addition is the option of automatic titration. This allows the isoelectric point to be determined by measuring zeta potential over a range of pH values.

There are several Beckman Coulter products to choose from, each with a range of optional extras, and Meritics will be happy to help you identify the right combination for your application. Our advisory resources include a telephone hotline and a team of field-based engineers, as well as our online information.

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